Sepsis Pathogenic Microorganism Detection Kit (Digital PCR)

A high-multiplex digital PCR assay designed for direct whole-blood detection of critical sepsis pathogens, offering superior sensitivity, rapid turnaround, and actionable results.

Why sepsis?

Bloodstream infection refers to a systemic infectious disease caused by bacteria, viruses, fungi, etc., invading the bloodstream, which can lead to sepsis. Sepsis refers to life-threatening organ dysfunction resulting from a dysregulated host response to infection. It has the characteristics of high morbidity, a high disability rate, and a high mortality rate, and is one of the causes of in-hospital death in patients in the intensive care unit.

The number of morbidities is high:

• Global: 49 million people/year (WHO, 2020)
• China: about 6.11 million people/year

High mortality rate:

• Global deaths: 11 million/year (WHO, 2020)
• Morbidity and mortality rate of more than 30%, plus cognitive dysfunction rate of 16.7%

Rapid onset:

• 58% increase in risk of death for each 6-hour delay in treatment
• Delaying treatment by two days increases the risk of death by 38.8 times

The gold standard – blood cultures:

• Low sensitivity:

- Broad-spectrum antibiotics are often misused, inhibiting colony growth

- Many pathogens cannot be cultured

• Longer incubation:

- More sets of blood are required

- Detection is empirical and susceptible to environmental influences

• Long testing time:

- 3–5 days — often too slow for effective diagnosis

• High sample volume requirement:

- 40–60 mL of blood needed → poor clinical accessibility

Comparison of Standard Diagnostic Methods vs. Digital PCR