Lyophilised Mastermix

For Research Use Only

Overview

Our lyophilized digital PCR mastermix is a probe-based formulation designed for high-precision droplet digital PCR (dPCR) applications. It contains all required PCR components - including buffer, dNTPs, Mg²⁺, and hot-start Taq polymerase - allowing users to simply add primers, probes, and templates. The lyophilised format enables room-temperature transport and storage, simplifying logistics and ensuring consistent performance.

Key Features

• Ready-to-use: Add primers, probes, and DNA - no additional reagents needed.
• Lyophilized for stability: Supports ambient-temperature shipping and storage.
• Compatible with dPCR and qPCR platforms.
• Designed for multiplex or contamination-free digital PCR assays.
• Works with RainSure systems (SG-series PCR amplifiers and Dscanner biochip readers).

How It Works

The mastermix partitions samples into tens of thousands of droplets on a digital PCR chip. Each droplet undergoes amplification, and fluorescence-positive droplets are quantified through Poisson statistics to determine absolute copy number with high precision.

Applications

Suitable for a wide range of nucleic-acid testing workflows, including:

• Genotyping & SNP detection
• Pathogen and viral detection
• Gene expression analysis (mRNA/miRNA)
• Copy number variation (CNV)
• Rare variant detection
• GMO analysis

Performance Highlights

• Consistent results: Match performance of our standard liquid dPCR mastermix
• Accurate quantification: Comparable copy number, clustering, and mutation-frequency results
• qPCR compatible: Shows amplification curves and sensitivity similar to leading commercial qPCR mixes
• Stable under stress: Maintains droplet quality and copy number after accelerated stability testing